Regarding Leghorn results, RE (%) increases and reaches the maximum value after 60 days of treatment for each considered metal. Results and discussionįungi from Genoa sediments increase the membrane absorption of Cu and Zn. To assess sediment contamination before and after the mycoremediation treatment, we considered chemical levels of reference L1 (the lowest chemical level of reference) and L2 (the highest chemical level of reference), and the evaluation of chemical hazard (HQ) for the chemical contaminants defined by the Italian Ministerial Decree 173/2016. Recovery efficiency (RE%) and difference recovery efficiency (DRE%) were calculated for each experiment: the first shows the absorption capability of the membrane-fungi consortium the second evidences only the fungal contribution to the metal absorption. Membranes were analyzed at 15, 30, and 60 days after inoculums, and sediments were analyzed after 60 days at the end of the experiment to verify metal contamination degree. Two plastic boxes were prepared (for each Port) with 5 kg of sediment in each box, employed for metal bioaccumulation using a sterile polyester membrane inoculated with fungi. Autochthonous fungi were isolated from each sediment and employed in mycoremediation tests. Materials and methodsĪctivity was carried out on port sediments from Genoa, Leghorn, Pisa, and Cagliari (Italy). In this work, we studied, developed, and tested a method for remediate marine-dredged sediments contaminated by heavy metals using native fungi and a microporous membrane, in order to achieve the sediment quality and allow their reuse. Dredged sediments are considered a waste by national laws, but recently the desire to consider them a resource has become widespread and remedies for their contamination are being searched to allow their reuse. Port-dredging activities produce large volumes of contaminated sediments.
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